Comparative biomonitoring of certain Dumre lakes tenable on cell cycle

Abstract

Dumre lakes with karst origin are mostly supplied by the rain water considered, until 30–40 years ago, as drinkable hydric resource. Nowadays, the loss of forests and soil degradation around them, likewise the intense agricultural practice and a number of anthropogenic activities carried out close to the lakes are very complex processes which may facilitate the eutrophication process. We evaluated the cytotoxic parameters as biomarkers of pollution from some lakes of Dumre. Three dry bulbs of Allium cepa were germinated in sample water, at room temperature. Controls were treated as well with tap water for the same period of time. The root tips were stained with aceto-orceine and then squashed and observed microscopically. The cytotoxity induced was compared with the value for the concomitant negative control using t-test. Only Belshi lake (23.7-52%) caused a significant reduction in the mitotic index compared with the negative control, which was indicative of lethal effects on test organism. Merhoja lake had the lowest mitodepressive value (43-13%). Comparing the phase indices, the separate phase durations were higher for the telophase index in samples of Merhoja and Seferan lakes showing a prompt cell division rate in that period. Decrease in the cell division process results from the effects of cytotoxity environmental chemicals which have the potential to cause adverse human health and environmental impact. We must consider necessary biomonitoring these lakes sited in a partially protected area.